Factors affecting the rate of catalase activity essay

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Factors affecting the rate of catalase activity essay

How to Write a Summary of an Article? To determine the effect of various factors on the rate of reaction between an enzyme and its substrate, and also to determine the optimal ranges under which the enzyme activity is maximized.

Also to determine whether saline and alcohol are inhibitors or activators Hypothesis: I predict that as the pH increases so the activity of the enzyme will increase until it reaches optimum pH range pH 7 because the enzyme is less denatured when it reaches the preferred pH level, and after this it will decrease because the active site will change in shape and it will no longer accept substrates.

I predict that as the concentration of enzyme increases, so the enzyme activities will increase because there is more enzyme to react with the substrates however when enzymes get saturated, the reaction will come to a plateau because eventually all the substrates will have enzymes to react with, and any extra will have no effect on the reaction whatsoever.

Use caution with hot and cold materials. Follow all safety procedures. Inquiry skills scientific Method: The dependant variable is time The independent variable is Peroxidase enzyme The controlled variables are PH, temperature, and concentration The reason to create this datum is so that we could make a comparison.

Without creating this action, it would be hard to see the effect of enzymes on the decomposition of peroxide. Inquiry skills data management: Quantitative Datum action - time in seconds Qualitative Datum action - extent of bubbling 10 0 20 1 30 1 40 2 50 1 60 1 Average: Quantitative Datum action — time in seconds Qualitative Datum action - extent of bubbling 10 3 20 2 30 2 40 2 50 1 60 0 Average 2 pH factor pH 3: Quantitative Datum action - time in seconds Qualitative Datum action - extent of bubbling 10 0 20 1 30 1 40 1 50 1 60 2 Average 1 pH factor pH 6: Quantitative Datum action - time in seconds Qualitative Datum action - extent of bubbling 10 1 20 2 30 2 40 3 50 4 60 4 Average 3 pH factor pH 7: Quantitative Datum action - time in seconds Qualitative Datum action - extent of bubbling 10 2 20 3 30 3 40 3 50 4 60 4 Average 3 pH factor pH 8: Quantitative Datum action - time in seconds Qualitative Datum action - extent of bubbling 10 3 20 2 30 2 40 2 50 2 60 1 Average 2 pH factor pH Quantitative Datum action - time in seconds Qualitative Datum action - extent of bubbling 10 2 20 1 30 1 40 1 50 0 60 0 Average 1 Concentration factor large pieces: Quantitative Datum action - time in seconds Qualitative Datum action - extent of bubbling 10 0 20 1 30 1 40 1 50 2 60 2 Average 1 Concentration factor medium pieces: Quantitative Datum action - time in seconds Qualitative Datum action - extent of bubbling 10 4 20 4 30 3 40 3 50 3 60 2 Average 3 Concentration factor small pieces: Quantitative Datum action - time in seconds Qualitative Datum action - extent of bubbling 10 1 20 1 30 1 40 1 50 0 60 0 Average 1 Knowledge and understanding Data Analysis: Knowledge and Understanding Concept Analysis: Enzymes are made of protein, depending on the structure of the amino acid, and the hydrogen and ionic bonds is what makes the difference between the two enzymes Catalase and Peroxidase.

My experiment results agrees with my hypothesis.The purpose of this experiment is to analyze the effect of multiple variables to determine if they have an effect on the rate at which catalase catalyzes H 2 0 2 decomposition.

Factors affecting the rate of catalase activity essay

Such factors include the re-using of the enzyme, increasing its surface area, the effect of temperature or its concentration. The effect the change of enzyme concentration had on the reaction time of catalase activity can be observed from the graph and table (a).

When the enzyme concentration was 25%, the rate of reaction was 22 seconds. However, when the enzyme was at % the reaction occurred within 9 seconds.

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This article throws light upon the six factors affecting the enzyme activity. The six factors are: (1) Concentration of Enzyme (2) Concentration of Substrate (3) Effect of Temperature (4) Effect of pH (5) Effect of Product Concentration and (6) Effect of Activators.

I’ve really noticed that credit restoration activity should be conducted with techniques. If not, chances are you’ll find yourself damaging your standing. Factors that Affect the Rate of Reaction of Peroxidase Purpose: To determine the effect of various factors on the rate of reaction between an enzyme and its substrate, and also Read more Discover the affects of PH on the enzyme catalase.

Composition of the culture medium, growth phase, and temperature play important roles in the sensitivity of Escherichia coli to H2O2. The medium and growth phase affected the sensitivity of the cells to H2O2 by modifying the amount of catalase synthesized by them, whereas the effect of temperature was due to the thermolability of the enzyme.

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